13 research outputs found
cDNA Cloning of Biologically Active Chicken Interleukin-18
Get PDFBy searching a chicken EST database, we identified a cDNA clone that appeared to contain the entire open
reading frame (ORF) of chicken interleukin-18 (ChIL-18). The encoded protein consists of 198 amino acids
and exhibits approximately 30% sequence identity to IL-18 of humans and various others mammals. Sequence
comparisons reveals a putative caspase-1 cleavage site at aspartic acid 29 of the primary translation product,
indicating that mature ChIL-18 might consist of 169 amino acids. Bacterially expressed ChIL-18 in which the
N-terminal 29 amino acids of the putative precursor molecule were replaced by a histidine tag induced the
synthesis of interferon-γ (IFN-γ) in cultured primary chicken spleen cells, indicating that the recombinant
protein is biologically active
Cloning, Expression and Bioactivity of Wild Ruddy Shelduck (Tadorna ferruginea) Interleukin-2
No full textOral delivery of novel therapeutics: development of a fowl adenovirus vector expressing chicken IL-2 and MGF
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Characterization of the First Nonmammalian T2 Cytokine Gene Cluster: The Cluster Contains Functional Single-Copy Genes for IL-3, IL-4, IL-13, and GM-CSF, a Gene for IL-5 That Appears to Be a Pseudogene, and a Gene Encoding Another Cytokinelike Transcript, KK34
No full textExpression of chicken interleukin-2 by turkey herpesvirus increases the immune response against Marek's disease virus but fails to increase protection against virulent challenge
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